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Natural mixtures of persistent organic pollutants (POP) increase weight gain, advance puberty, and induce changes in gene expression associated with steroid hormones and obesity in female zebrafish.

Identifieur interne : 000388 ( Main/Exploration ); précédent : 000387; suivant : 000389

Natural mixtures of persistent organic pollutants (POP) increase weight gain, advance puberty, and induce changes in gene expression associated with steroid hormones and obesity in female zebrafish.

Auteurs : Jan L. Lyche [Norvège] ; Rasoul Nourizadeh-Lillabadi ; Camilla Almaas ; Benedicte Stavik ; Vidar Berg ; Janneche Utne Sk Re ; Peter Alestr M ; Erik Ropstad

Source :

RBID : pubmed:20526952

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English descriptors

Abstract

In the present study, developmental and reproductive effects of lifelong exposure to environmental relevant concentrations of two natural mixtures of persistent organic pollutants (POP) were investigated using classical and molecular methods in a controlled zebrafish model. The mixtures used were extracted from burbot (Lota lota) liver originating from freshwater systems in Norway: one mixture with high levels and one mixture with background levels of polybrominated diphenyl ethers (PBDE), polychlorinated biphenyls (PCB), and dichlorodiphenyltrichloroethane metabolites (DDT). The concentration of POP measured in the zebrafish ranged from levels detected in wild fish from Lake Mjøsa to concentrations reported in human and wildlife populations, indicating that the experimental fish were exposed to concentrations comparable with wild fish. Phenotypic effects observed in both exposure groups included earlier onset of puberty, increased male/female sex ratio, and differences in body weight at 5 mo of age. Interestingly, genome-wide transcription profiling showed changes in regulation of genes involved in endocrine signaling and growth. The transcriptomics changes include key regulator genes for steroid hormone functions (ncoa3), and growth (c/ebp, ncoa3). The effects observed in the experimental zebrafish model raise the question whether chemical pollution represents a risk to reproductive health of wild fish inhabitating the freshwater system.

DOI: 10.1080/15287394.2010.481618
PubMed: 20526952


Affiliations:


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<keywords scheme="KwdEn" xml:lang="en">
<term>Animals (MeSH)</term>
<term>Artemia (chemistry)</term>
<term>Complex Mixtures (analysis)</term>
<term>Complex Mixtures (toxicity)</term>
<term>DDT (analysis)</term>
<term>DDT (toxicity)</term>
<term>Female (MeSH)</term>
<term>Fresh Water (MeSH)</term>
<term>Gene Expression (drug effects)</term>
<term>Gene Expression Profiling (MeSH)</term>
<term>Halogenated Diphenyl Ethers (analysis)</term>
<term>Halogenated Diphenyl Ethers (toxicity)</term>
<term>In Situ Hybridization (MeSH)</term>
<term>Liver (chemistry)</term>
<term>Liver (metabolism)</term>
<term>Obesity (chemically induced)</term>
<term>Oligonucleotide Array Sequence Analysis (MeSH)</term>
<term>Organic Chemicals (analysis)</term>
<term>Organic Chemicals (toxicity)</term>
<term>Polychlorinated Biphenyls (analysis)</term>
<term>Polychlorinated Biphenyls (toxicity)</term>
<term>RNA (biosynthesis)</term>
<term>RNA (genetics)</term>
<term>Reverse Transcriptase Polymerase Chain Reaction (MeSH)</term>
<term>Sex Ratio (MeSH)</term>
<term>Sexual Maturation (drug effects)</term>
<term>Steroids (biosynthesis)</term>
<term>Water Pollutants, Chemical (analysis)</term>
<term>Water Pollutants, Chemical (toxicity)</term>
<term>Weight Gain (drug effects)</term>
<term>Zebrafish (MeSH)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr">
<term>ARN (biosynthèse)</term>
<term>ARN (génétique)</term>
<term>Analyse de profil d'expression de gènes (MeSH)</term>
<term>Animaux (MeSH)</term>
<term>Artemia (composition chimique)</term>
<term>Composés chimiques organiques (analyse)</term>
<term>Composés chimiques organiques (toxicité)</term>
<term>DDT (analyse)</term>
<term>DDT (toxicité)</term>
<term>Danio zébré (MeSH)</term>
<term>Eau douce (MeSH)</term>
<term>Expression des gènes (effets des médicaments et des substances chimiques)</term>
<term>Femelle (MeSH)</term>
<term>Foie (composition chimique)</term>
<term>Foie (métabolisme)</term>
<term>Hybridation in situ (MeSH)</term>
<term>Maturation sexuelle (effets des médicaments et des substances chimiques)</term>
<term>Mélanges complexes (analyse)</term>
<term>Mélanges complexes (toxicité)</term>
<term>Obésité (induit chimiquement)</term>
<term>Polluants chimiques de l'eau (analyse)</term>
<term>Polluants chimiques de l'eau (toxicité)</term>
<term>Polychlorobiphényles (analyse)</term>
<term>Polychlorobiphényles (toxicité)</term>
<term>Prise de poids (effets des médicaments et des substances chimiques)</term>
<term>RT-PCR (MeSH)</term>
<term>Sexe-ratio (MeSH)</term>
<term>Stéroïdes (biosynthèse)</term>
<term>Séquençage par oligonucléotides en batterie (MeSH)</term>
<term>Éthers de polyhalogénophényle (analyse)</term>
<term>Éthers de polyhalogénophényle (toxicité)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en">
<term>Complex Mixtures</term>
<term>DDT</term>
<term>Halogenated Diphenyl Ethers</term>
<term>Organic Chemicals</term>
<term>Polychlorinated Biphenyls</term>
<term>Water Pollutants, Chemical</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en">
<term>RNA</term>
<term>Steroids</term>
</keywords>
<keywords scheme="MESH" qualifier="analyse" xml:lang="fr">
<term>Composés chimiques organiques</term>
<term>DDT</term>
<term>Mélanges complexes</term>
<term>Polluants chimiques de l'eau</term>
<term>Polychlorobiphényles</term>
<term>Éthers de polyhalogénophényle</term>
</keywords>
<keywords scheme="MESH" qualifier="biosynthèse" xml:lang="fr">
<term>ARN</term>
<term>Stéroïdes</term>
</keywords>
<keywords scheme="MESH" qualifier="chemically induced" xml:lang="en">
<term>Obesity</term>
</keywords>
<keywords scheme="MESH" qualifier="chemistry" xml:lang="en">
<term>Artemia</term>
<term>Liver</term>
</keywords>
<keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr">
<term>Artemia</term>
<term>Foie</term>
</keywords>
<keywords scheme="MESH" qualifier="drug effects" xml:lang="en">
<term>Gene Expression</term>
<term>Sexual Maturation</term>
<term>Weight Gain</term>
</keywords>
<keywords scheme="MESH" qualifier="effets des médicaments et des substances chimiques" xml:lang="fr">
<term>Expression des gènes</term>
<term>Maturation sexuelle</term>
<term>Prise de poids</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en">
<term>RNA</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr">
<term>ARN</term>
</keywords>
<keywords scheme="MESH" qualifier="induit chimiquement" xml:lang="fr">
<term>Obésité</term>
</keywords>
<keywords scheme="MESH" qualifier="metabolism" xml:lang="en">
<term>Liver</term>
</keywords>
<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr">
<term>Foie</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="toxicity" xml:lang="en">
<term>Complex Mixtures</term>
<term>DDT</term>
<term>Halogenated Diphenyl Ethers</term>
<term>Organic Chemicals</term>
<term>Polychlorinated Biphenyls</term>
<term>Water Pollutants, Chemical</term>
</keywords>
<keywords scheme="MESH" qualifier="toxicité" xml:lang="fr">
<term>Composés chimiques organiques</term>
<term>DDT</term>
<term>Mélanges complexes</term>
<term>Polluants chimiques de l'eau</term>
<term>Polychlorobiphényles</term>
<term>Éthers de polyhalogénophényle</term>
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<term>Animals</term>
<term>Female</term>
<term>Fresh Water</term>
<term>Gene Expression Profiling</term>
<term>In Situ Hybridization</term>
<term>Oligonucleotide Array Sequence Analysis</term>
<term>Reverse Transcriptase Polymerase Chain Reaction</term>
<term>Sex Ratio</term>
<term>Zebrafish</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr">
<term>Analyse de profil d'expression de gènes</term>
<term>Animaux</term>
<term>Danio zébré</term>
<term>Eau douce</term>
<term>Femelle</term>
<term>Hybridation in situ</term>
<term>RT-PCR</term>
<term>Sexe-ratio</term>
<term>Séquençage par oligonucléotides en batterie</term>
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<front>
<div type="abstract" xml:lang="en">In the present study, developmental and reproductive effects of lifelong exposure to environmental relevant concentrations of two natural mixtures of persistent organic pollutants (POP) were investigated using classical and molecular methods in a controlled zebrafish model. The mixtures used were extracted from burbot (Lota lota) liver originating from freshwater systems in Norway: one mixture with high levels and one mixture with background levels of polybrominated diphenyl ethers (PBDE), polychlorinated biphenyls (PCB), and dichlorodiphenyltrichloroethane metabolites (DDT). The concentration of POP measured in the zebrafish ranged from levels detected in wild fish from Lake Mjøsa to concentrations reported in human and wildlife populations, indicating that the experimental fish were exposed to concentrations comparable with wild fish. Phenotypic effects observed in both exposure groups included earlier onset of puberty, increased male/female sex ratio, and differences in body weight at 5 mo of age. Interestingly, genome-wide transcription profiling showed changes in regulation of genes involved in endocrine signaling and growth. The transcriptomics changes include key regulator genes for steroid hormone functions (ncoa3), and growth (c/ebp, ncoa3). The effects observed in the experimental zebrafish model raise the question whether chemical pollution represents a risk to reproductive health of wild fish inhabitating the freshwater system.</div>
</front>
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